How does a solid phase extraction column work?

How does a solid phase extraction column work?

Solid phase extraction uses the difference of affinity between an analyte and interferents, present in a liquid matrix, for a solid phase (sorbent). This affinity allows the separation of the target analyte from the interferents.

What is a SPE column?

Pre-packed solid phase extraction columns, with a variety of sorbents or resins, for vacuum, positive pressure, or centrifugation processing methods; in a variety of particle sizes and bed weights.

What is the basic principle of solid phase extraction?

The basic principle of SPE is the partitioning of compounds between two phases of solid and liquid and there must be greater affinity for the solid phase than for the sample matrix. The compounds retained on the solid phase can be removed by eluting solvent with a greater affinity for the analytes.

What is matrix in solid phase extraction?

Matrix solid phase dispersion (MSPD) is a technique widely used in sample preparation for the extraction of chemical constituents from fruits and plants, as well as for the extraction of organic contaminants from food and environmental samples.

What is automated solid phase extraction?

Solid-phase extraction, also referred to as SPE system, is a separation process that bridges the gap between the sample collection and analysis. The solid phase extraction principle is based upon and very similar to chromatographic processes.

How do you choose solid phase extraction?

Selectivity of SPE In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.

What is the difference between liquid phase extraction and solid phase extraction?

Solid-phase extraction (SPE) is a sample preparation technique by which compounds that are dissolved or suspended in a liquid matrix are extracted according to their physical and chemical properties. Liquid-liquid extraction (LLE) employs water-immiscible solvents to extract analytes from aqueous solutions.

What is automated SPE?

How do I use SPE?

To perform normal-phase chromatography with SPE cartridges, use a step gradient of nonpolar solvents with a polar packing material.

1. Condition the cartridge with six to ten hold-up volumes of non-polar solvent, usually the same solvent in which the sample is dissolved.
2. Load the sample solution onto the cartridge bed.